1S1Z

Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 to 500 nanoseconds

Summary for 1S1Z

• Entry DOI: 10.2210/pdb1s1z/pdb
• Related: 1S1Y
• Descriptor: Photoactive Yellow Protein, 4'-HYDROXYCINNAMIC ACID (3 entities in total)
• Functional Keywords: room temperature, time-resolved, photoreceptor
• Biological source: Halorhodospira halophila
• Total number of polymer chains: 1
• Total formula weight: 14051.75

Authors

Anderson, S.,Srajer, V.,Pahl, R.,Rajagopal, S.,Schotte, F.,Anfinrud, P.,Wulff, M.,Moffat, K. (deposition date: 2004-01-07, release date: 2004-06-15, Last modification date: 2021-10-27)

Primary citation

Anderson, S.,Srajer, V.,Pahl, R.,Rajagopal, S.,Schotte, F.,Anfinrud, P.,Wulff, M.,Moffat, K.
Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein
Structure, 12:1039-1045, 2004

PubMed Abstract: We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen bond network to the chromophore. Although structural change occurs within a few nanoseconds on and around the chromophore, it takes milliseconds for a distinct pattern of tertiary structural change to fully progress through the entire molecule, thus generating the putative signaling state. Remarkably, the coupling between the chromophore conformation and the tertiary structure of this small protein is not tight: there are leads and lags between changes in the conformation of the chromophore and the protein tertiary structure.

PubMed: 15274923
DOI: 10.1016/j.str.2004.04.008

Experimental method

X-RAY DIFFRACTION (1.6 Å)