1S1Y

Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds

1S1Y の概要

• エントリーDOI: 10.2210/pdb1s1y/pdb
• 関連するPDBエントリー: 1S1Z
• 分子名称: Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID (3 entities in total)
• 機能のキーワード: room temperature, time-resolved, photoreceptor
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14051.75

構造登録者

Anderson, S.,Srajer, V.,Pahl, R.,Rajagopal, S.,Schotte, F.,Anfinrud, P.,Wulff, M.,Moffat, K. (登録日: 2004-01-07, 公開日: 2004-06-15, 最終更新日: 2021-10-27)

主引用文献

Anderson, S.,Srajer, V.,Pahl, R.,Rajagopal, S.,Schotte, F.,Anfinrud, P.,Wulff, M.,Moffat, K.
Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein
Structure, 12:1039-1045, 2004

PubMed Abstract: We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen bond network to the chromophore. Although structural change occurs within a few nanoseconds on and around the chromophore, it takes milliseconds for a distinct pattern of tertiary structural change to fully progress through the entire molecule, thus generating the putative signaling state. Remarkably, the coupling between the chromophore conformation and the tertiary structure of this small protein is not tight: there are leads and lags between changes in the conformation of the chromophore and the protein tertiary structure.

PubMed: 15274923
DOI: 10.1016/j.str.2004.04.008

実験手法

X-RAY DIFFRACTION (1.6 Å)