1S0Y

The structure of trans-3-chloroacrylic acid dehalogenase, covalently inactivated by the mechanism-based inhibitor 3-bromopropiolate at 2.3 Angstrom resolution

Summary for 1S0Y

• Entry DOI: 10.2210/pdb1s0y/pdb
• Descriptor: alpha-subunit of trans-3-chloroacrylic acid dehalogenase, beta-subunit of trans-3-chloroacrylic acid dehalogenase, MALONIC ACID, ... (4 entities in total)
• Functional Keywords: dehalogenase, tautomerase family, covalent modification, inhibition, michael addition, dehalogenation mechanism, malonyl inhibitor, lyase
• Biological source: Pseudomonas pavonaceae; More
• Total number of polymer chains: 12
• Total formula weight: 97431.17

Authors

de Jong, R.M.,Brugman, W.,Poelarends, G.J.,Whitman, C.P.,Dijkstra, B.W. (deposition date: 2004-01-05, release date: 2004-02-24, Last modification date: 2023-08-23)

• Primary citation: de Jong, R.M.,Brugman, W.,Poelarends, G.J.,Whitman, C.P.,Dijkstra, B.W.; The X-ray structure of trans-3-chloroacrylic acid dehalogenase reveals a novel hydration mechanism in the tautomerase superfamily; J.Biol.Chem., 279:11546-11552, 2004; PubMed: 14701869; DOI: 10.1074/jbc.M311966200

Experimental method

X-RAY DIFFRACTION (2.3 Å)