1S03

The Structure of a Ribosomal Protein S8/spc Operon mRNA Complex

1S03 の概要

• エントリーDOI: 10.2210/pdb1s03/pdb
• 分子名称: 47-MER, 30S ribosomal protein S8, ZINC ION, ... (4 entities in total)
• 機能のキーワード: protein-rna complex, ribosomal, spc operon, transcription-rna complex, transcription/rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 59374.76

構造登録者

Merianos, H.J.,Wang, J.,Moore, P.B. (登録日: 2003-12-29, 公開日: 2004-05-25, 最終更新日: 2023-08-23)

主引用文献

Merianos, H.J.,Wang, J.,Moore, P.B.
The structure of a ribosomal protein S8/spc operon mRNA complex.
RNA, 10:954-964, 2004

PubMed Abstract: In bacteria, translation of all the ribosomal protein cistrons in the spc operon mRNA is repressed by the binding of the product of one of them, S8, to an internal sequence at the 5' end of the L5 cistron. The way in which the first two genes of the spc operon are regulated, retroregulation, is mechanistically distinct from translational repression by S8 of the genes from L5 onward. A 2.8 A resolution crystal structure has been obtained of Escherichia coli S8 bound to this site. Despite sequence differences, the structure of this complex is almost identical to that of the S8/helix 21 complex seen in the small ribosomal subunit, consistent with the hypothesis that autogenous regulation of ribosomal protein synthesis results from conformational similarities between mRNAs and rRNAs. S8 binding must repress the translation of its own mRNA by inhibiting the formation of a ribosomal initiation complex at the start of the L5 cistron.

PubMed: 15146079
DOI: 10.1261/rna.7030704

実験手法

X-RAY DIFFRACTION (2.7 Å)