1RZL

RICE NONSPECIFIC LIPID TRANSFER PROTEIN

1RZL の概要

• エントリーDOI: 10.2210/pdb1rzl/pdb
• 分子名称: NONSPECIFIC LIPID TRANSFER PROTEIN, SULFATE ION, 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: lipid transport, alpha-helical structure
• 由来する生物種: Oryza sativa (rice)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9457.88

構造登録者

Lee, J.Y.,Min, K.S.,Cha, H.,Shin, D.H.,Hwang, K.Y.,Suh, S.W. (登録日: 1997-10-09, 公開日: 1998-12-16, 最終更新日: 2023-08-09)

主引用文献

Lee, J.Y.,Min, K.,Cha, H.,Shin, D.H.,Hwang, K.Y.,Suh, S.W.
Rice non-specific lipid transfer protein: the 1.6 A crystal structure in the unliganded state reveals a small hydrophobic cavity.
J.Mol.Biol., 276:437-448, 1998

PubMed Abstract: This study describes the high-resolution X-ray structure of the non-specific lipid transfer protein (ns-LTP) from rice seeds in the unliganded state. The model has been refined to a crystallographic R-factor of 0.186 for 8.0 to 1.6 A data (with Fo > 2 sigma F). It accounts for all 91 amino acid residues, 68 water molecules, one sulfate ion, and two molecules of 3-[cyclohexylamino]-1-propanesulfonic acid. The root-mean-square deviations from ideal bond lengths and angles are 0.017 A and 1.76 degrees, respectively. The overall fold of rice ns-LTP is very similar to that of maize ns-LTP. A superposition of 91 common C alpha atoms in rice and maize ns-LTPs, both in the unliganded state, gives a root-mean-square deviation of 1.2 A. Large structural differences from the crystal structure of maize ns-LTP are observed in two regions: the loop between two alpha-helices H1 and H2, where one residue deletion (Gln21 of maize sequence) occurs, and the C-terminal region around Tyr79. The C-terminal region of rice protein is somewhat collapsed into the hydrophobic cavity. As a consequence, its hydrophobic cavity is considerably smaller than that of maize protein (144 A3 versus 408 A3 for van der Waals cavity volumes), despite a high level of sequence identity (79%) between them. In the rice ns-LTP structure, the side-chain of Arg44 partially blocks the mouth of the cavity, while the side-chain of Ile81 effectively closes the other end by protruding into the cavity. And the side-chain of Tyr79 divides the cavity into two parts, with the larger part being shielded from the solvent. The present study illuminates the structure-function relationship of rice ns-LTP and allows a detailed structural comparison with other plant ns-LTPs.

PubMed: 9512714
DOI: 10.1006/jmbi.1997.1550

実験手法

X-RAY DIFFRACTION (1.6 Å)