1RZD

X-RAY ANALYSIS OF METAL SUBSTITUTED HUMAN CARBONIC ANHYDRASE II DERIVATIVES

1RZD の概要

• エントリーDOI: 10.2210/pdb1rzd/pdb
• 分子名称: CARBONIC ANHYDRASE II, MANGANESE (II) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: lyase(oxo-acid)
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm : P00918
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29308.86

構造登録者

Hakansson, K.,Wehnert, A.,Liljas, A. (登録日: 1993-05-25, 公開日: 1993-10-31, 最終更新日: 2024-02-14)

主引用文献

Hakansson, K.,Wehnert, A.,Liljas, A.
X-ray analysis of metal-substituted human carbonic anhydrase II derivatives.
Acta Crystallogr.,Sect.D, 50:93-100, 1994

PubMed Abstract: Metal-substituted crystals of human carbonic anhydrase II belonging to space group P2(1) with cell dimensions a = 42.7, b = 41.7, c = 73.0 A and beta = 104.6 degrees were analyzed crystallographically. The resolution limit ranged from 1.82 to 1.92 A with high completeness (86.2-90.7%). Cobalt(II)-substituted carbonic anhydrase has a tetrahedral coordination around the metal both at pH 6 and pH 7.8, similar to the native zinc enzyme. In contrast, the catalytically inactive copper(II), nickel(II) and manganese(II) derivatives showed increased coordination number around the metal ion. Whereas the copper is best described as penta-coordinated, the nickel and manganese are best described as hexa-coordinated. The results are briefly compared with spectroscopic observations and our current view on carbonic anhydrase catalysis.

PubMed: 15299481
DOI: 10.1107/S0907444993008790

実験手法

X-RAY DIFFRACTION (1.9 Å)