1RZ4

Crystal Structure of Human eIF3k

1RZ4 の概要

• エントリーDOI: 10.2210/pdb1rz4/pdb
• 分子名称: Eukaryotic translation initiation factor 3 subunit 11, SULFATE ION (3 entities in total)
• 機能のキーワード: heat analogous motif, winged-helix, biosynthetic protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q9UBQ5
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26532.21

構造登録者

Wei, Z.,Zhang, P.,Zhou, Z.,Gong, W. (登録日: 2003-12-23, 公開日: 2004-09-21, 最終更新日: 2024-10-30)

主引用文献

Wei, Z.,Zhang, P.,Zhou, Z.,Cheng, Z.,Wan, M.,Gong, W.
Crystal structure of human eIF3k, the first structure of eIF3 subunits
J.Biol.Chem., 279:34983-34990, 2004

PubMed Abstract: eIF3k, the smallest subunit of eukaryotic initiation factor 3 (eIF3), interacts with several other subunits of eIF3 and the 40 S ribosomal subunit. eIF3k is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. Interestingly, eIF3k does not exist in some species of yeast. Thus, eIF3k may play a unique regulatory role in higher organisms. Here we report the crystal structure of human eIF3k, the first high-resolution structure of an eIF3 component. This novel structure contains two distinct domains, a HEAT (named for Huntington, elongation factor 3, A subunit of protein phosphatase 2A, target of rapamycin) repeat-like HAM (HEAT analogous motif) domain and a winged-helix-like WH domain. Through structural comparison and sequence conservation analysis, we show that eIF3k has three putative protein-binding surfaces and has potential RNA binding activity. The structure provides key information for understanding the structure and function of the eIF3 complex.

PubMed: 15180986
DOI: 10.1074/jbc.M405158200

実験手法

X-RAY DIFFRACTION (2.1 Å)