1RYK

Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;

Replaces:  1JYG

Summary for 1RYK

• Entry DOI: 10.2210/pdb1ryk/pdb
• Related: 1JYG
• Descriptor: Protein yjbJ (1 entity in total)
• Functional Keywords: alpha protein, structural genomics, protein structure initiative, ocsp, nesg, psi, northeast structural genomics consortium, unknown function
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 8339.26

Authors

Pineda-Lucena, A.,Liao, J.,Wu, B.,Yee, A.,Cort, J.R.,Kennedy, M.A.,Edwards, A.M.,Arrowsmith, C.H.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2003-12-22, release date: 2004-02-24, Last modification date: 2024-05-22)

Primary citation

Pineda-Lucena, A.,Liao, J.,Wu, B.,Yee, A.,Cort, J.R.,Kennedy, M.A.,Edwards, A.M.,Arrowsmith, C.H.
An NMR approach to structural proteomics.
Proteins, 47:572-574, 2002

PubMed Abstract: The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

PubMed: 11854485
DOI: 10.1002/prot.10120.abs

Experimental method

SOLUTION NMR