1RXA

CRYSTAL STRUCTURE OF R(CCCCGGGG) IN TWO DISTINCT LATTICES

1RXA の概要

• エントリーDOI: 10.2210/pdb1rxa/pdb
• 分子名称: RNA (5'-R(*CP*CP*CP*CP*GP*GP*GP*G)-3') (1 entity in total)
• 機能のキーワード: a-rna, double helix, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2556.59

構造登録者

Portmann, S.,Usman, N.,Egli, M. (登録日: 1995-05-05, 公開日: 1996-10-20, 最終更新日: 2024-02-14)

主引用文献

Portmann, S.,Usman, N.,Egli, M.
The crystal structure of r(CCCCGGGG) in two distinct lattices.
Biochemistry, 34:7569-7575, 1995

PubMed Abstract: The structure of the RNA octamer r(C4G4) has been determined in two different crystal forms. The conformations of the RNA duplex in the rhombohedral and hexagonal lattices display only minimal deviations, demonstrating that the RNA double helix is considerably less deformable than the DNA double helix. For the first time the crystal structures of an A-RNA and an A-DNA with identical sequence can now be compared. The large number of ordered water molecules observed in the minor groove of the RNA duplex suggests that an important contribution to its higher rigidity derives from the improved hydration due to the presence of the 2'-hydroxyl groups. Our finding that the conformation of the RNA double helix is virtually unaffected by different crystal packing modes provides evidence that proteins may not alter the conformation of RNA stem regions in a significant way.

PubMed: 7779802
DOI: 10.1021/bi00023a002

実験手法

X-RAY DIFFRACTION (2.9 Å)