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1RWD

Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings

1M2Y」から置き換えられました
1RWD の概要
エントリーDOI10.2210/pdb1rwd/pdb
関連するPDBエントリー1M2Y
NMR情報BMRB: 5926
分子名称Rubredoxin (1 entity in total)
機能のキーワードresidual dipolar couplings, structural genomics, psi, protein structure initiative, southeast collaboratory for structural genomics, secsg, electron transport
由来する生物種Pyrococcus furiosus
タンパク質・核酸の鎖数1
化学式量合計5863.47
構造登録者
Tian, F.,Valafar, H.,Prestegard, J.H.,Southeast Collaboratory for Structural Genomics (SECSG) (登録日: 2003-12-16, 公開日: 2003-12-23, 最終更新日: 2024-05-22)
主引用文献Tian, F.,Valafar, H.,Prestegard, J.H.
A Dipolar Coupling Based Strategy for Simultaneous Resonance Assignment and Structure Determination of Protein Backbones
J.Am.Chem.Soc., 123:11791-11796, 2001
Cited by
PubMed Abstract: A new approach for simultaneous protein backbone resonance assignment and structure determination by NMR is introduced. This approach relies on recent advances in high-resolution NMR spectroscopy that allow observation of anisotropic interactions, such as dipolar couplings, from proteins partially aligned in field ordered media. Residual dipolar couplings are used for both geometric information and a filter in the assembly of residues in a sequential manner. Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C. Given the acceleration possible with partial 13C enrichment, the protocol described should provide a very rapid route to protein structure determination. This is critical for the structural genomics initiative where protein expression and structural determination in a high-throughput manner will be needed.
PubMed: 11716736
DOI: 10.1021/ja011806h
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 1rwd
検証レポート(詳細版)ダウンロードをダウンロード

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件を2026-04-29に公開中

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