1RWD
Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings
「1M2Y」から置き換えられました1RWD の概要
| エントリーDOI | 10.2210/pdb1rwd/pdb |
| 関連するPDBエントリー | 1M2Y |
| NMR情報 | BMRB: 5926 |
| 分子名称 | Rubredoxin (1 entity in total) |
| 機能のキーワード | residual dipolar couplings, structural genomics, psi, protein structure initiative, southeast collaboratory for structural genomics, secsg, electron transport |
| 由来する生物種 | Pyrococcus furiosus |
| タンパク質・核酸の鎖数 | 1 |
| 化学式量合計 | 5863.47 |
| 構造登録者 | Tian, F.,Valafar, H.,Prestegard, J.H.,Southeast Collaboratory for Structural Genomics (SECSG) (登録日: 2003-12-16, 公開日: 2003-12-23, 最終更新日: 2024-05-22) |
| 主引用文献 | Tian, F.,Valafar, H.,Prestegard, J.H. A Dipolar Coupling Based Strategy for Simultaneous Resonance Assignment and Structure Determination of Protein Backbones J.Am.Chem.Soc., 123:11791-11796, 2001 Cited by PubMed Abstract: A new approach for simultaneous protein backbone resonance assignment and structure determination by NMR is introduced. This approach relies on recent advances in high-resolution NMR spectroscopy that allow observation of anisotropic interactions, such as dipolar couplings, from proteins partially aligned in field ordered media. Residual dipolar couplings are used for both geometric information and a filter in the assembly of residues in a sequential manner. Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C. Given the acceleration possible with partial 13C enrichment, the protocol described should provide a very rapid route to protein structure determination. This is critical for the structural genomics initiative where protein expression and structural determination in a high-throughput manner will be needed. PubMed: 11716736DOI: 10.1021/ja011806h 主引用文献が同じPDBエントリー |
| 実験手法 | SOLUTION NMR |
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