1RUT

Complex of LMO4 LIM domains 1 and 2 with the ldb1 LID domain

1RUT の概要

• エントリーDOI: 10.2210/pdb1rut/pdb
• 関連するPDBエントリー: 1m3v
• 分子名称: Fusion protein of Lmo4 protein and LIM domain-binding protein 1, ZINC ION (3 entities in total)
• 機能のキーワード: b-tandem zipper, lim domain, fusion protein, protein binding
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Nucleus : P70662
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20457.28

構造登録者

Deane, J.E.,Ryan, D.P.,Maher, M.J.,Kwan, A.H.Y.,Bacca, M.,Mackay, J.P.,Guss, J.M.,Visvader, J.E.,Matthews, J.M. (登録日: 2003-12-11, 公開日: 2004-10-12, 最終更新日: 2024-05-29)

主引用文献

Deane, J.E.,Ryan, D.P.,Sunde, M.,Maher, M.J.,Guss, J.M.,Visvader, J.E.,Matthews, J.M.
Tandem LIM domains provide synergistic binding in the LMO4:Ldb1 complex
Embo J., 23:3589-3598, 2004

PubMed Abstract: Nuclear LIM-only (LMO) and LIM-homeodomain (LIM-HD) proteins have important roles in cell fate determination, organ development and oncogenesis. These proteins contain tandemly arrayed LIM domains that bind the LIM interaction domain (LID) of the nuclear adaptor protein LIM domain-binding protein-1 (Ldb1). We have determined a high-resolution X-ray crystal structure of LMO4, a putative breast oncoprotein, in complex with Ldb1-LID, providing the first example of a tandem LIM:Ldb1-LID complex and the first structure of a type-B LIM domain. The complex possesses a highly modular structure with Ldb1-LID binding in an extended manner across both LIM domains of LMO4. The interface contains extensive hydrophobic and electrostatic interactions and multiple backbone-backbone hydrogen bonds. A mutagenic screen of Ldb1-LID, assessed by yeast two-hybrid and competition ELISA analysis, identified key features at the interface and revealed that the interaction is tolerant to mutation. These combined properties provide a mechanism for the binding of Ldb1 to numerous LMO and LIM-HD proteins. Furthermore, the modular extended interface may form a general mode of binding to tandem LIM domains.

PubMed: 15343268
DOI: 10.1038/sj.emboj.7600376

実験手法

X-RAY DIFFRACTION (1.3 Å)