1RTB

CRYSTAL STRUCTURE DISPOSITION OF THYMIDYLIC ACID TETRAMER IN COMPLEX WITH RIBONUCLEASE A

1RTB の概要

• エントリーDOI: 10.2210/pdb1rtb/pdb
• 分子名称: RIBONUCLEASE A (1 entity in total)
• 機能のキーワード: hydrolase(nucleic acid, rna)
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13708.33

構造登録者

Birdsall, D.L.,McPherson, A. (登録日: 1992-08-28, 公開日: 1993-10-31, 最終更新日: 2024-10-30)

主引用文献

Birdsall, D.L.,McPherson, A.
Crystal structure disposition of thymidylic acid tetramer in complex with ribonuclease A.
J.Biol.Chem., 267:22230-22236, 1992

PubMed Abstract: The crystal structure of ribonuclease A with bound thymidylic acid tetramer is reported at 2.5-A resolution. The diffusion of the tetramer into native orthorhombic crystals of the ribonuclease allows for the formation of a structurally stable complex where the single-stranded nucleic acid enters and leaves the enzyme's catalytic region in a persistent 5'-3' direction. The binding of the tetramer to the enzyme's surface is facilitated and mediated by electrostatic interactions between basic protein residues and nucleotide phosphates. Two pyrimidine nucleotides are bound to the enzyme's active site in a manner similar to that observed for other complexes between ribonuclease A and nucleic acid oligomers.

PubMed: 1429575

実験手法

X-RAY DIFFRACTION (2.5 Å)