1RPV

HIV-1 REV PROTEIN (RESIDUES 34-50)

1RPV の概要

• エントリーDOI: 10.2210/pdb1rpv/pdb
• 分子名称: HIV-1 REV PROTEIN (1 entity in total)
• 機能のキーワード: human immunodeficiency virus-1, rev response element, transcription regulation protein
• 由来する生物種: Human immunodeficiency virus type 1 (CLONE 12)
• 細胞内の位置: Host nucleus, host nucleolus: P12485
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2401.75

構造登録者

Scanlon, M.J.,Fairlie, D.P.,Craik, D.J.,Englebretsen, D.R.,West, M.L. (登録日: 1995-05-04, 公開日: 1995-10-15, 最終更新日: 2022-03-02)

主引用文献

Scanlon, M.J.,Fairlie, D.P.,Craik, D.J.,Englebretsen, D.R.,West, M.L.
NMR solution structure of the RNA-binding peptide from human immunodeficiency virus (type 1) Rev.
Biochemistry, 34:8242-8249, 1995

PubMed Abstract: NMR spectroscopy has been used to solve the three-dimensional solution structure of a minimal RNA-binding domain of the Rev protein from the human immunodeficiency virus (type 1), an essential regulatory protein for viral replication. The presence of 10 arginine residues in the 17-residue peptide Rev34-50 caused significant problems in assignment of the NMR spectra. To improve spectral resolution, the peptide was synthesized with an alanine replacing a nonessential arginine and with selectively 15N-labeled residues. Contrary to Chou-Fasman modeling predictions an alpha-helix was detected in both water and 20% trifluoroethanol (TFE) and was found to span residues that constitute the RNA-binding and nuclear-localizing domains of Rev. The sequence-specific information provided by the NMR data gives a full description of the solution conformation of Rev34-50 which serves as a template for investigating binding of the peptide to RNA from the Rev response element (RRE). Preliminary modeling suggests that the helix can fit neatly into the expanded major groove of the RRE where interactions between the peptide side chains and the RNA can be identified. These data may aid the construction of a suitable pharmacophore model for the rational design of molecules that block Rev-RNA binding and inhibit HIV replication.

PubMed: 7599117
DOI: 10.1021/bi00026a005

実験手法

SOLUTION NMR