1RPR

THE STRUCTURE OF COLE1 ROP IN SOLUTION

1RPR の概要

• エントリーDOI: 10.2210/pdb1rpr/pdb
• 分子名称: ROP (1 entity in total)
• 機能のキーワード: transcription regulation
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14474.08

構造登録者

Eberle, W.,Pastore, A.,Klaus, W.,Sander, C.,Roesch, P. (登録日: 1991-10-09, 公開日: 1994-01-31, 最終更新日: 2024-05-22)

主引用文献

Eberle, W.,Pastore, A.,Sander, C.,Rosch, P.
The structure of ColE1 rop in solution.
J.Biomol.NMR, 1:71-82, 1991

PubMed Abstract: The structure of the ColE1 repressor of primer (rop) protein in solution was determined from the proton nuclear magnetic resonance data by a combined use of distance geometry and restrained molecular dynamics calculations. A set of structures was determined with low internal energy and virtually no violations of the experimental distance restraints. Rop forms homodimers: Two helical hairpins are arranged as an antiparallel four helix bundle with a left-handed rope-like twist of the helix axes and with left-handed bundle topology. The very compact packing of the side chains in the helix interfaces of the rop coiled-coil structure may well account for its high stability. Overall, the solution structure is highly similar to the recently determined X-ray structure (Banner, D.W., Kokkinidis, M. and Tsernoglou, D. (1987) J. Mol. Biol., 196, 657-675), although there are minor differences in regions where packing forces appear to influence the crystal structure.

PubMed: 1841691
DOI: 10.1007/BF01874570

実験手法

SOLUTION NMR