1RPL

2.3 ANGSTROMS CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF DNA POLYMERASE BETA

1RPL の概要

• エントリーDOI: 10.2210/pdb1rpl/pdb
• 分子名称: DNA POLYMERASE BETA (2 entities in total)
• 機能のキーワード: nucleotidyltransferase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Nucleus: P06766
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29145.98

構造登録者

Davies II, J.F.,Almassy, R.J. (登録日: 1994-10-25, 公開日: 1995-01-26, 最終更新日: 2024-02-14)

主引用文献

Davies 2nd., J.F.,Almassy, R.J.,Hostomska, Z.,Ferre, R.A.,Hostomsky, Z.
2.3 A crystal structure of the catalytic domain of DNA polymerase beta.
Cell(Cambridge,Mass.), 76:1123-1133, 1994

PubMed Abstract: The crystal structure of the catalytic domain of rat DNA polymerase beta (pol beta) has been determined at 2.3 A resolution and refined to an R factor of 0.22. The mixed alpha/beta protein has three subdomains arranged in an overall U shape reminiscent of other polymerase structures. The folding topology of pol beta, however, is unique. Two divalent metals bind near three aspartic acid residues implicated in the catalytic activity. In the presence of Mn2+ and dTTP, interpretable electron density is seen for two metals and the triphosphate, but not the deoxythymidine moiety. The principal interaction of the triphosphate moiety is with the bound divalent metals.

PubMed: 8137427
DOI: 10.1016/0092-8674(94)90388-3

実験手法

X-RAY DIFFRACTION (2.3 Å)