1ROP

STRUCTURE OF THE COL*E1 ROP PROTEIN AT 1.7 ANGSTROMS RESOLUTION

1ROP の概要

• エントリーDOI: 10.2210/pdb1rop/pdb
• 分子名称: ROP PROTEIN (2 entities in total)
• 機能のキーワード: transcription regulation
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7237.04

構造登録者

Kokkinidis, M.,Banner, D.W.,Tsernoglou, D. (登録日: 1991-04-02, 公開日: 1992-07-15, 最終更新日: 2024-02-14)

主引用文献

Banner, D.W.,Kokkinidis, M.,Tsernoglou, D.
Structure of the ColE1 rop protein at 1.7 A resolution
J.Mol.Biol., 196:657-675, 1987

PubMed Abstract: Structural details of the Rop protein from plasmid ColE1 are presented, with a description of the X-ray crystal structure determination and refinement at a nominal resolution of 1.7 A. The 63 amino acid protein is a dimer. Each monomer consists almost entirely of two alpha helices, the whole molecule forming a highly regular four-alpha-helix bundle. This may be approximated by a four-stranded rope with a radius of 7.0 A, a left-handed helical twist and a pitch of 172.5 A. The packing constraints for this novel type of coiled-coil structure are given. The protein acts in the control of plasmid replication via regulation of an RNA-RNA interaction in a manner not yet understood in atomic detail.

PubMed: 3681971
DOI: 10.1016/0022-2836(87)90039-8

実験手法

X-RAY DIFFRACTION (1.7 Å)