1RLG
Molecular basis of Box C/D RNA-protein interaction: co-crystal structure of the Archaeal sRNP intiation complex
Summary for 1RLG
| Entry DOI | 10.2210/pdb1rlg/pdb |
| Descriptor | 25-MER, 50S ribosomal protein L7Ae (2 entities in total) |
| Functional Keywords | protein-rna, structural protein-rna complex, structural protein/rna |
| Biological source | Archaeoglobus fulgidus |
| Cellular location | Cytoplasm : O29494 |
| Total number of polymer chains | 4 |
| Total formula weight | 43083.79 |
| Authors | Moore, T.,Zhang, Y.,Fenley, M.O.,Li, H. (deposition date: 2003-11-25, release date: 2004-06-01, Last modification date: 2024-02-14) |
| Primary citation | Moore, T.,Zhang, Y.,Fenley, M.O.,Li, H. Molecular Basis of Box C/D RNA-Protein Interactions; Cocrystal Structure of Archaeal L7Ae and a Box C/D RNA. STRUCTURE, 12:807-818, 2004 Cited by PubMed Abstract: We have determined and refined a crystal structure of the initial assembly complex of archaeal box C/D sRNPs comprising the Archaeoglobus fulgidus (AF) L7Ae protein and a box C/D RNA. The box C/D RNA forms a classical kink-turn (K-turn) structure and the resulting protein-RNA complex serves as a distinct platform for recruitment of the fibrillarin-Nop5p complex. The cocrystal structure confirms previously proposed secondary structure of the box C/D RNA that includes a protruded U, a UU mismatch, and two sheared tandem GA base pairs. Detailed structural comparisons of the AF L7Ae-box C/D RNA complex with previously determined crystal structures of L7Ae homologs in complex with functionally distinct K-turn RNAs revealed a set of remarkably conserved principles in protein-RNA interactions. These analyses provide a structural basis for interpreting the functional roles of the box C/D sequences in directing specific assembly of box C/D sRNPs. PubMed: 15130473DOI: 10.1016/j.str.2004.02.033 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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