1RLG

Molecular basis of Box C/D RNA-protein interaction: co-crystal structure of the Archaeal sRNP intiation complex

1RLG の概要

• エントリーDOI: 10.2210/pdb1rlg/pdb
• 分子名称: 25-MER, 50S ribosomal protein L7Ae (2 entities in total)
• 機能のキーワード: protein-rna, structural protein-rna complex, structural protein/rna
• 由来する生物種: Archaeoglobus fulgidus
• 細胞内の位置: Cytoplasm : O29494
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 43083.79

構造登録者

Moore, T.,Zhang, Y.,Fenley, M.O.,Li, H. (登録日: 2003-11-25, 公開日: 2004-06-01, 最終更新日: 2024-02-14)

主引用文献

Moore, T.,Zhang, Y.,Fenley, M.O.,Li, H.
Molecular Basis of Box C/D RNA-Protein Interactions; Cocrystal Structure of Archaeal L7Ae and a Box C/D RNA.
STRUCTURE, 12:807-818, 2004

PubMed Abstract: We have determined and refined a crystal structure of the initial assembly complex of archaeal box C/D sRNPs comprising the Archaeoglobus fulgidus (AF) L7Ae protein and a box C/D RNA. The box C/D RNA forms a classical kink-turn (K-turn) structure and the resulting protein-RNA complex serves as a distinct platform for recruitment of the fibrillarin-Nop5p complex. The cocrystal structure confirms previously proposed secondary structure of the box C/D RNA that includes a protruded U, a UU mismatch, and two sheared tandem GA base pairs. Detailed structural comparisons of the AF L7Ae-box C/D RNA complex with previously determined crystal structures of L7Ae homologs in complex with functionally distinct K-turn RNAs revealed a set of remarkably conserved principles in protein-RNA interactions. These analyses provide a structural basis for interpreting the functional roles of the box C/D sequences in directing specific assembly of box C/D sRNPs.

PubMed: 15130473
DOI: 10.1016/j.str.2004.02.033

実験手法

X-RAY DIFFRACTION (2.7 Å)