1RL1

Solution structure of human Sgt1 CS domain

1RL1 の概要

• エントリーDOI: 10.2210/pdb1rl1/pdb
• NMR情報: BMRB: 6012
• 分子名称: Suppressor of G2 allele of SKP1 homolog (1 entity in total)
• 機能のキーワード: beta sandwich, 7 beta strands, similar to p23, lacking last beta strand seen in p23, protein degradation
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13124.12

構造登録者

Lee, Y.-T.,Jacob, J.,Michowski, W.,Nowotny, M.,Kuznicki, J.,Chazin, W.J. (登録日: 2003-11-24, 公開日: 2004-05-04, 最終更新日: 2024-05-22)

主引用文献

Lee, Y.-T.,Jacob, J.,Michowski, W.,Nowotny, M.,Kuznicki, J.,Chazin, W.J.
Human Sgt1 Binds HSP90 through the CHORD-Sgt1 Domain and Not the Tetratricopeptide Repeat Domain
J.Biol.Chem., 279:16511-16517, 2004

PubMed Abstract: Sgt1 has been identified as a subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes and is also implicated in plant disease resistance. Sgt1 has two putative HSP90 binding domains, a tetratricopeptide repeat and a p23-like CHORD and Sgt1 (CS) domain. Using NMR spectroscopy, we show that only the CS domain of human Sgt1 physically interacts with HSP90. The tetratricopeptide repeat domain does not bind to either HSP90 or HSP70. Determination of the three-dimensional structure showed that the Sgt1-CS domain shares the same beta-sandwich fold as p23 but lacks the last highly conserved beta-strand in p23. Analysis of the structures of Sgt1-CS and p23 revealed a similar charge distribution on one of two opposing surfaces that suggests that it is the binding region for HSP90 in Sgt1. Although ATP is absolutely required for p23 binding to HSP90, Sgt1 binds to HSP90 also in the absence of the non-hydrolyzable analog ATPgammaS. Our findings suggest the CS domain is a binding module for HSP90 distinct from p23-like domains, which implies that Sgt1 and related proteins function in recruiting heat shock protein activities to multiprotein assemblies.

PubMed: 14761955
DOI: 10.1074/jbc.M400215200

実験手法

SOLUTION NMR