1RKB

The structure of adrenal gland protein AD-004

1RKB の概要

• エントリーDOI: 10.2210/pdb1rkb/pdb
• 分子名称: Protein AD-004, SULFATE ION, LITHIUM ION, ... (4 entities in total)
• 機能のキーワード: five-stranded parallel beta-sheet flanked by 7 alpha-helices, transferase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus, nucleoplasm: Q9Y3D8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20696.70

構造登録者

Ren, H.,Liang, Y.,Bennett, M.,Su, X.D. (登録日: 2003-11-21, 公開日: 2005-01-11, 最終更新日: 2024-03-13)

主引用文献

Ren, H.,Wang, L.,Bennett, M.,Liang, Y.,Zheng, X.,Lu, F.,Li, L.,Nan, J.,Luo, M.,Eriksson, S.,Zhang, C.,Su, X.D.
The crystal structure of human adenylate kinase 6: An adenylate kinase localized to the cell nucleus
Proc.Natl.Acad.Sci.Usa, 102:303-308, 2005

PubMed Abstract: Adenylate kinases (AKs) play important roles in nucleotide metabolism in all organisms and in cellular energetics by means of phosphotransfer networks in eukaryotes. The crystal structure of a human AK named AK6 was determined by in-house sulfur single-wavelength anomalous dispersion phasing methods and refined to 2.0-A resolution with a free R factor of 21.8%. Sequence analyses revealed that human AK6 belongs to a distinct subfamily of AKs present in all eukaryotic organisms sequenced so far. Enzymatic assays show that human AK6 has properties similar with other AKs, particularly with AK5. Fluorescence microscopy showed that human AK6 is localized predominantly to the nucleus of HeLa cells. The identification of a nuclear-localized AK sheds light on nucleotide metabolism in the nucleus and the energetic communication between mitochondria and nucleus by means of phosphotransfer networks.

PubMed: 15630091
DOI: 10.1073/pnas.0407459102

実験手法

X-RAY DIFFRACTION (2 Å)