1RJZ

Mhc Class I Natural Mutant H-2Kbm8 Heavy Chain Complexed With beta-2 Microglobulin and Herpies Simplex Virus Mutant Glycoprotein B Peptide

1RJZ の概要

• エントリーDOI: 10.2210/pdb1rjz/pdb
• 分子名称: H-2 class I histocompatibility antigen, K-B alpha chain, Beta-2-microglobulin, Glycoprotein B, ... (4 entities in total)
• 機能のキーワード: mhc, class i, virus, tcr, herpes, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 89961.07

構造登録者

Miley, M.J.,Messaoudi, I.,Nikolich-Zugich, J.,Fremont, D.H. (登録日: 2003-11-20, 公開日: 2004-12-14, 最終更新日: 2024-11-13)

主引用文献

Miley, M.J.,Messaoudi, I.,Metzner, B.M.,Wu, Y.,Nikolich-Zugich, J.,Fremont, D.H.
Structural Basis for the Restoration of TCR Recognition of an MHC Allelic Variant by Peptide Secondary Anchor Substitution
J.Exp.Med., 200:1445-1454, 2004

PubMed Abstract: Major histocompatibility complex (MHC) class I variants H-2K(b) and H-2K(bm8) differ primarily in the B pocket of the peptide-binding groove, which serves to sequester the P2 secondary anchor residue. This polymorphism determines resistance to lethal herpes simplex virus (HSV-1) infection by modulating T cell responses to the immunodominant glycoprotein B(498-505) epitope, HSV8. We studied the molecular basis of these effects and confirmed that T cell receptors raised against K(b)-HSV8 cannot recognize H-2K(bm8)-HSV8. However, substitution of Ser(P2) to Glu(P2) (peptide H2E) reversed T cell receptor (TCR) recognition; H-2K(bm8)-H2E was recognized whereas H-2K(b)-H2E was not. Insight into the structural basis of this discrimination was obtained by determining the crystal structures of all four MHC class I molecules in complex with bound peptide (pMHCs). Surprisingly, we find no concerted pMHC surface differences that can explain the differential TCR recognition. However, a correlation is apparent between the recognition data and the underlying peptide-binding groove chemistry of the B pocket, revealing that secondary anchor residues can profoundly affect TCR engagement through mechanisms distinct from the alteration of the resting state conformation of the pMHC surface.

PubMed: 15557346
DOI: 10.1084/jem.20040217

実験手法

X-RAY DIFFRACTION (2.6 Å)