1RJI

Solution Structure of BmKX, a novel potassium channel blocker from the Chinese Scorpion Buthus martensi Karsch

1RJI の概要

• エントリーDOI: 10.2210/pdb1rji/pdb
• NMR情報: BMRB: 6037
• 分子名称: potassium channel toxin KX (1 entity in total)
• 機能のキーワード: 3-10 helix, beta sheet, toxin
• 細胞内の位置: Secreted: Q7Z0H4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3333.88

構造登録者

Cai, Z.,Wu, J.,Xu, Y.,Wang, C.-G.,Chi, C.-W.,Shi, Y. (登録日: 2003-11-19, 公開日: 2003-12-09, 最終更新日: 2024-10-23)

主引用文献

Wang, C.-G.,Cai, Z.,Lu, W.,Wu, J.,Xu, Y.,Shi, Y.,Chi, C.-W.
A novel short-chain peptide BmKX from the Chinese scorpion Buthus martensi karsch, sequencing, gene cloning and structure determination
Toxicon, 45:309-319, 2005

PubMed Abstract: Scorpion venom is a rich source of bioactive peptides. From the venom of Chinese scorpion Buthus martensi Karsch (BmK), a novel short chain peptide BmKX of 31-amino acid residues was purified, and its amino acid sequence and gene structure were determined. The gene of BmKX was composed of two exons interrupted by an 86-bp intron at the codon-7 upstream of the mature peptide. Although its gene structure is similar to those of other known scorpion toxins, its amino acid sequence, especially the cysteine framework, is different from those of all other known subfamilies of short-chain scorpion toxins. The solution structure of BmKX, determined with two-dimensional NMR spectroscopy, shows that BmKX also forms a typical cysteine-stabilized alpha/beta scaffold adopted by most short-chain scorpion toxins, consisting of a short 3(10)-helix and a two-stranded antiparallel beta-sheet, and the short N-terminal segment forms a pseudo-strand of the beta-sheet. However, the orientation between the helix and the beta-sheet is significantly different from the others, which might be the reason for its unique but still unclear physiological function.

PubMed: 15683869
DOI: 10.1016/j.toxicon.2004.11.014

実験手法

SOLUTION NMR