1RIP

RIBOSOMAL PROTEIN S17: CHARACTERIZATION OF THE THREE-DIMENSIONAL STRUCTURE BY 1H-AND 15N-NMR

1RIP の概要

• エントリーDOI: 10.2210/pdb1rip/pdb
• 分子名称: RIBOSOMAL PROTEIN S17 (1 entity in total)
• 機能のキーワード: ribosomal protein
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9555.31

構造登録者

Golden, B.L.,Hoffman, D.W.,Ramakrishnan, V.,White, S.W. (登録日: 1993-08-17, 公開日: 1993-10-31, 最終更新日: 2024-05-22)

主引用文献

Golden, B.L.,Hoffman, D.W.,Ramakrishnan, V.,White, S.W.
Ribosomal protein S17: characterization of the three-dimensional structure by 1H and 15N NMR.
Biochemistry, 32:12812-12820, 1993

PubMed Abstract: The structure of ribosomal protein S17 from Bacillus stearothermophilus was investigated by two-dimensional homonuclear and heteronuclear magnetic resonance spectroscopy. The 1H and 15N chemical shift assignments are largely complete, and a preliminary structural characterization is presented. The protein consists of five beta-strands that form a single antiparallel beta-sheet with Greek-key topology. The beta-strands are connected by several extended loops, and two of these contain residue types that are frequently seen in the RNA-binding sites of proteins. Additionally, two point mutations that affect antibiotic resistance, translational fidelity, and ribosome assembly are located in these two regions of the protein. Since these potential RNA-binding sites are distributed over a large surface of the protein, it appears that the molecule may interact with several regions of 16S rRNA.

PubMed: 8251502
DOI: 10.1021/bi00210a033

実験手法

SOLUTION NMR