1RI7

crystal structure of a protein in the LRP/ASNC family from the hyperthermophilic archaeon Pyrococcus sp. OT3

1RI7 の概要

• エントリーDOI: 10.2210/pdb1ri7/pdb
• 分子名称: Putative transcriptional regulator (2 entities in total)
• 機能のキーワード: lrp/asnc family, transcription, dna binding protein
• 由来する生物種: Pyrococcus horikoshii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19151.22

構造登録者

Koike, H.,Suzuki, M. (登録日: 2003-11-16, 公開日: 2004-03-09, 最終更新日: 2024-03-13)

主引用文献

Koike, H.,Ishijima, S.A.,Clowney, L.,Suzuki, M.
The archaeal feast/famine regulatory protein: Potential roles of its assembly forms for regulating transcription
Proc.Natl.Acad.Sci.USA, 101:2840-2845, 2004

PubMed Abstract: The classification feast/famine regulatory proteins (FFRPs) encompasses archaeal DNA-binding proteins with Escherichia coli transcription factors, the leucine-responsive regulatory protein and the asparagine synthase C gene product. In this paper, we describe two forms of the archaeal FFRP FL11 (pot0434017), both assembled from dimers. When crystallized, a helical cylinder is formed with six dimers per turn. In contrast, in solution, disks are formed, most likely consisting of four dimers each; an observation by cryoelectron microscopy. Whereas each dimer binds a 13-bp sequence, different forms will discriminate between promoters, based on the numbers of repeating 13-bp sequences, and types of linkers inserted between them, which are either of 7-8 or approximately 18 bp. The amino acid sequences of these FFRPs are designed to form the same type of 3D structures, and the transition between their assembly forms is regulated by interaction with small molecules. These considerations lead us to propose a possible mechanism for regulating a number of genes by varying assembly forms and by combining different FFRPs into these assemblies, responding to environmental changes.

PubMed: 14976242
DOI: 10.1073/pnas.0400109101

実験手法

X-RAY DIFFRACTION (2.7 Å)