1RHP

CRYSTAL STRUCTURE OF RECOMBINANT HUMAN PLATELET FACTOR 4

1RHP の概要

• エントリーDOI: 10.2210/pdb1rhp/pdb
• 分子名称: PLATELET FACTOR 4 (2 entities in total)
• 機能のキーワード: platelet factor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P02776
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 31128.78

構造登録者

Chen, L.,Zhang, X. (登録日: 1994-09-16, 公開日: 1994-11-30, 最終更新日: 2024-11-20)

主引用文献

Zhang, X.,Chen, L.,Bancroft, D.P.,Lai, C.K.,Maione, T.E.
Crystal structure of recombinant human platelet factor 4.
Biochemistry, 33:8361-8366, 1994

PubMed Abstract: The crystal structure of human platelet factor 4 (PF4) has been solved to a resolution of 2.4 A by molecular replacement and refined to an R-factor of 24.1%. The structure consists of four polypeptide chains which form a tetrameric unit. N-terminal residues, previously defined as a random coil or extended loop region, form antiparallel beta-sheet-like structures that form noncovalent associations between dimers. These antiparallel beta-sheet-like structures are positioned lateral to the beta-bilayer motif and stabilize the tetrameric unit. A positively charged ring of lysine and arginine side chains encircles the PF4 tetramer sphere, presenting multiple potential sites and orientations for heparin binding. The electrostatic interactions of multiply charged amino acid side chains and hydrogen bonding interactions at the AB/CD dimer interface serve to stabilize the tetrameric structure further.

PubMed: 8031770
DOI: 10.1021/bi00193a025

実験手法

X-RAY DIFFRACTION (2.4 Å)