1RGS

REGULATORY SUBUNIT OF CAMP DEPENDENT PROTEIN KINASE

1RGS の概要

• エントリーDOI: 10.2210/pdb1rgs/pdb
• 分子名称: CAMP DEPENDENT PROTEIN KINASE, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE (2 entities in total)
• 機能のキーワード: regulatory subunit, kinase
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33152.27

構造登録者

Su, Y.,Dostmann, W.R.G.,Herberg, F.W.,Durick, K.,Xuong, N.-H.,Ten Eyck, L.,Taylor, S.S.,Varughese, K.I. (登録日: 1995-06-21, 公開日: 1996-12-07, 最終更新日: 2024-02-14)

主引用文献

Su, Y.,Dostmann, W.R.,Herberg, F.W.,Durick, K.,Xuong, N.H.,Ten Eyck, L.,Taylor, S.S.,Varughese, K.I.
Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains.
Science, 269:807-813, 1995

PubMed Abstract: In the molecular scheme of living organisms, adenosine 3',5'-monophosphate (cyclic AMP or cAMP) has been a universal second messenger. In eukaryotic cells, the primary receptors for cAMP are the regulatory subunits of cAMP-dependent protein kinase. The crystal structure of a 1-91 deletion mutant of the type I alpha regulatory subunit was refined to 2.8 A resolution. Each of the two tandem cAMP binding domains provides an extensive network of hydrogen bonds that buries the cyclic phosphate and the ribose between two beta strands that are linked by a short alpha helix. Each adenine base stacks against an aromatic ring that lies outside the beta barrel. This structure provides a molecular basis for understanding how cAMP binds cooperatively to its receptor protein, thus mediating activation of the kinase.

PubMed: 7638597

実験手法

X-RAY DIFFRACTION (2.8 Å)