1RGP

GTPASE-ACTIVATION DOMAIN FROM RHOGAP

1RGP の概要

• エントリーDOI: 10.2210/pdb1rgp/pdb
• 分子名称: RHOGAP (2 entities in total)
• 機能のキーワード: g-protein, gap, signal-transduction
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q07960
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27444.47

構造登録者

Barrett, T.,Xiao, B.,Dodson, E.J.,Dodson, G.,Ludbrook, S.B.,Nurmahomed, K.,Gamblin, S.J.,Musacchio, A.,Smerdon, S.J.,Eccleston, J.F. (登録日: 1996-12-05, 公開日: 1997-10-15, 最終更新日: 2024-02-14)

主引用文献

Barrett, T.,Xiao, B.,Dodson, E.J.,Dodson, G.,Ludbrook, S.B.,Nurmahomed, K.,Gamblin, S.J.,Musacchio, A.,Smerdon, S.J.,Eccleston, J.F.
The structure of the GTPase-activating domain from p50rhoGAP.
Nature, 385:458-461, 1997

PubMed Abstract: Members of the Rho family of small G proteins transduce signals from plasma-membrane receptors and control cell adhesion, motility and shape by actin cytoskeleton formation. They also activate other kinase cascades. Like all other GTPases, Rho proteins act as molecular switches, with an active GTP-bound form and an inactive GDP-bound form. The active conformation is promoted by guanine-nucleotide exchange factors, and the inactive state by GTPase-activating proteins (GAPs) which stimulate the intrinsic GTPase activity of small G proteins. Rho-specific GAP domains are found in a wide variety of large, multi-functional proteins. Here we report the crystal structure of an active 242-residue C-terminal fragment of human p50rhoGAP. The structure is an unusual arrangement of nine alpha-helices, the core of which includes a four-helix bundle. Residues conserved across the rhoGAP family are largely confined to one face of this bundle, which may be an interaction site for target G proteins. In particular, we propose that Arg 85 and Asn 194 are involved in binding G proteins and enhancing GTPase activity.

PubMed: 9009196
DOI: 10.1038/385458a0

実験手法

X-RAY DIFFRACTION (2 Å)