1RGO

Structural Basis for Recognition of the mRNA Class II AU-Rich Element by the Tandem Zinc Finger Domain of TIS11d

1RGO の概要

• エントリーDOI: 10.2210/pdb1rgo/pdb
• NMR情報: BMRB: 6005
• 分子名称: RNA (5'-R(*UP*UP*AP*UP*UP*UP*AP*UP*U)-3'), Butyrate response factor 2, ZINC ION (3 entities in total)
• 機能のキーワード: tis11 ttp tristetraprolin butyrate response factor erf nup475 zfp zn zinc finger rna ss single-stranded are utr tandem intercalation intercalate specific, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus (Potential): P47974
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11202.88

構造登録者

Hudson, B.P.,Martinez-Yamout, M.A.,Dyson, H.J.,Wright, P.E. (登録日: 2003-11-12, 公開日: 2004-03-02, 最終更新日: 2024-05-22)

主引用文献

Hudson, B.P.,Martinez-Yamout, M.A.,Dyson, H.J.,Wright, P.E.
Recognition of the mRNA AU-rich element by the zinc finger domain of TIS11d.
NAT.STRUCT.MOL.BIOL., 11:257-264, 2004

PubMed Abstract: The tandem zinc finger (TZF) domain of the protein TIS11d binds to the class II AU-rich element (ARE) in the 3' untranslated region (3' UTR) of target mRNAs and promotes their deadenylation and degradation. The NMR structure of the TIS11d TZF domain bound to the RNA sequence 5'-UUAUUUAUU-3' comprises a pair of novel CCCH fingers of type CX(8)CX(5)CX(3)H separated by an 18-residue linker. The two TIS11d zinc fingers bind in a symmetrical fashion to adjacent 5'-UAUU-3' subsites on the single-stranded RNA via a combination of electrostatic and hydrogen-bonding interactions, with intercalative stacking between conserved aromatic side chains and the RNA bases. Sequence specificity in RNA recognition is achieved by a network of intermolecular hydrogen bonds, mostly between TIS11d main-chain functional groups and the Watson-Crick edges of the bases. The TIS11d structure provides insights into the RNA-binding functions of this large family of CCCH zinc finger proteins.

PubMed: 14981510
DOI: 10.1038/nsmb738

実験手法

SOLUTION NMR