1RFD

ANTI-COCAINE ANTIBODY M82G2

1RFD の概要

• エントリーDOI: 10.2210/pdb1rfd/pdb
• 関連するPDBエントリー: 1Q72 1QYG
• 分子名称: Fab M82G2, Light Chain, Fab M82G2, Heavy Chain (3 entities in total)
• 機能のキーワード: anti-cocaine antibody, fab, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47403.63

構造登録者

Pozharski, E.,Hewagama, A.,Shanafelt, A.B.,Petsko, G.A.,Ringe, D. (登録日: 2003-11-07, 公開日: 2003-11-18, 最終更新日: 2024-11-20)

主引用文献

Pozharski, E.,Moulin, A.,Hewagama, A.,Shanafelt, A.B.,Petsko, G.A.,Ringe, D.
Diversity in hapten recognition: structural study of an anti-cocaine antibody M82G2.
J.Mol.Biol., 349:570-582, 2005

PubMed Abstract: Antibodies against cocaine and other drugs of abuse are the basis for diagnostic tests for the presence of those drugs in human serum. The 1.7A resolution crystal structure of the anti-cocaine monoclonal antibody M82G2 in complex with cocaine is presented. This structure determination was undertaken to establish the stereochemical features in the antibody binding site that confer specificity for cocaine, and as part of an ongoing project to understand the rules that govern molecular recognition. The cocaine-binding site can be characterized topologically as a narrow groove on the protein surface. The antibody utilizes water-mediated hydrogen bonding, and cation-pi and stacking (pi-pi) interactions to provide specificity. Comparison with the previously published structure of the anti-cocaine antibody GNC92H2 shows that binding of a small ligand can be achieved in diverse ways, both in terms of a binding site structure/topology and protein-ligand interactions.

PubMed: 15885702
DOI: 10.1016/j.jmb.2005.03.080

実験手法

X-RAY DIFFRACTION (2.09 Å)