1REG

CRYSTAL STRUCTURE OF THE T4 REGA TRANSLATIONAL REGULATOR PROTEIN AT 1.9 ANGSTROMS RESOLUTION

1REG の概要

• エントリーDOI: 10.2210/pdb1reg/pdb
• 分子名称: T4 REGA (2 entities in total)
• 機能のキーワード: translational regulator protein
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29286.30

構造登録者

Kang, C.,Rich, A. (登録日: 1995-01-11, 公開日: 1996-01-01, 最終更新日: 2024-02-14)

主引用文献

Kang, C.,Chan, R.,Berger, I.,Lockshin, C.,Green, L.,Gold, L.,Rich, A.
Crystal structure of the T4 regA translational regulator protein at 1.9 A resolution.
Science, 268:1170-1173, 1995

PubMed Abstract: The translational regulator protein regA is encoded by the T4 bacteriophage and binds to a region of messenger RNA (mRNA) that includes the initiator codon. RegA is unusual in that it represses the translation of about 35 early T4 mRNAs but does not affect nearly 200 other mRNAs. The crystal structure of regA was determined at 1.9 A resolution; the protein was shown to have an alpha-helical core and two regions with antiparallel beta sheets. One of these beta sheets has four antiparallel strands and has some sequence homology to RNP-1 and RNP-2, which are believed to be RNA-binding motifs and are found in a number of known RNA-binding proteins. Structurally guided mutants may help to uncover the basis for this variety of RNA interaction.

PubMed: 7761833

実験手法

X-RAY DIFFRACTION (1.9 Å)