1RC7

Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution

1RC7 の概要

• エントリーDOI: 10.2210/pdb1rc7/pdb
• 関連するPDBエントリー: 1I4S 1JFZ 1O0W 1RC5
• 分子名称: 5'-R(*GP*GP*CP*GP*CP*GP*CP*GP*CP*C)-3', Ribonuclease III, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: ribonuclease iii, ds-rna, rna interference, endonucleolytic cleavage, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Aquifex aeolicus
• 細胞内の位置: Cytoplasm (By similarity): O67082
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 38968.40

構造登録者

Blaszczyk, J.,Gan, J.,Ji, X. (登録日: 2003-11-03, 公開日: 2004-03-30, 最終更新日: 2023-08-30)

主引用文献

Blaszczyk, J.,Gan, J.,Tropea, J.E.,Court, D.L.,Waugh, D.S.,Ji, X.
Noncatalytic Assembly of Ribonuclease III with Double-Stranded RNA.
Structure, 12:457-466, 2004

PubMed Abstract: Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) endonucleases. The simplest bacterial enzyme contains an endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure and gene expression in either of two ways: as a dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex with dsRNA, revealing a noncatalytic assembly. The major differences between the two functional forms of RNase III.dsRNA are the conformation of the protein and the orientation and location of dsRNA. The flexibility of a 7 residue linker between the endoND and dsRBD enables the transition between these two forms.

PubMed: 15016361
DOI: 10.1016/j.str.2004.02.004

実験手法

X-RAY DIFFRACTION (2.15 Å)