1R9J

Transketolase from Leishmania mexicana

1R9J の概要

• エントリーDOI: 10.2210/pdb1r9j/pdb
• 分子名称: transketolase, CALCIUM ION, THIAMINE DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: 3 domains, each of the alpha/beta type, thiamine diphosphate binding domain, transferase
• 由来する生物種: Leishmania mexicana mexicana
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 145338.80

構造登録者

Veitch, N.J.,Mauger, D.A.,Cazzulo, J.J.,Lindqvist, Y.,Barrett, M.P. (登録日: 2003-10-30, 公開日: 2004-11-09, 最終更新日: 2023-08-23)

主引用文献

Veitch, N.J.,Maugeri, D.A.,Cazzulo, J.J.,Lindqvist, Y.,Barrett, M.P.
Transketolase from Leishmania mexicana has a dual subcellular localization.
Biochem.J., 382:759-767, 2004

PubMed Abstract: Transketolase has been characterized in Leishmania mexicana. A gene encoding this enzyme was identified and cloned. The gene was expressed in Escherichia coli and the protein was purified and characterized. An apparent K(m) of 2.75 mM for ribose 5-phosphate was determined. X-ray crystallography was used to determine the three-dimensional structure of the enzyme to a resolution of 2.2 A (1 A identical with 0.1 nm). The C-terminus of the protein contains a type-1 peroxisome-targeting signal, suggestive of a possible glycosomal subcellular localization. Subcellular localization experiments performed with promastigote forms of the parasite revealed that the protein was predominantly cytosolic, although a significant component of the total activity was associated with the glycosomes. Transketolase is thus the first enzyme of the nonoxidative branch of the pentose phosphate pathway whose presence has been demonstrated in a peroxisome-like organelle.

PubMed: 15149284
DOI: 10.1042/BJ20040459

実験手法

X-RAY DIFFRACTION (2.22 Å)