1R9G

Three-dimensional Structure of YaaE from Bacillus subtilis

1R9G の概要

• エントリーDOI: 10.2210/pdb1r9g/pdb
• 分子名称: Hypothetical protein yaaE (2 entities in total)
• 機能のキーワード: triad amidotransferase, glutaminase, alpha/beta protein, cofactor biosynthesis, transferase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47846.70

構造登録者

Bauer, J.A.,Bennett, E.M.,Begley, T.P.,Ealick, S.E. (登録日: 2003-10-29, 公開日: 2004-01-27, 最終更新日: 2024-10-30)

主引用文献

Bauer, J.A.,Bennett, E.M.,Begley, T.P.,Ealick, S.E.
Three-dimensional Structure of YaaE from Bacillus subtilis, a Glutaminase Implicated in Pyridoxal-5'-phosphate Biosynthesis.
J.Biol.Chem., 279:2704-2711, 2004

PubMed Abstract: The structure of YaaE from Bacillus subtilis was determined at 2.5-A resolution. YaaE is a member of the triad glutamine aminotransferase family and functions in a recently identified alternate pathway for the biosynthesis of vitamin B(6). Proposed active residues include conserved Cys-79, His-170, and Glu-172. YaaE shows similarity to HisH, a glutaminase involved in histidine biosynthesis. YaaD associates with YaaE. A homology model of this protein was constructed. YaaD is predicted to be a (beta/alpha)(8) barrel on the basis of sequence comparisons. The predicted active site includes highly conserved residues 211-216 and 233-235. Finally, a homology model of a putative YaaD-YaaE complex was prepared using the structure of HisH-F as a model. This model predicts that the ammonia molecule generated by YaaE is channeled through the center of the YaaD barrel to the putative YaaD active site.

PubMed: 14585832
DOI: 10.1074/jbc.M310311200

実験手法

X-RAY DIFFRACTION (2.5 Å)