1R88

The crystal structure of Mycobacterium tuberculosis MPT51 (FbpC1)

1R88 の概要

• エントリーDOI: 10.2210/pdb1r88/pdb
• 分子名称: MPT51/MPB51 antigen (2 entities in total)
• 機能のキーワード: mycobacterium tuberculosis, alfa/beta hydrolase fold, antigen 85, mpt51, fbpc1, immune system
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Secreted: P0A4V6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58913.25

構造登録者

Wilson, R.A.,Maughan, W.N.,Kremer, L.,Besra, G.S.,Futterer, K. (登録日: 2003-10-23, 公開日: 2003-12-30, 最終更新日: 2023-11-08)

主引用文献

Wilson, R.A.,Maughan, W.N.,Kremer, L.,Besra, G.S.,Futterer, K.
The structure of Mycobacterium tuberculosis MPT51 (FbpC1) defines a new family of non-catalytic alpha/beta hydrolases.
J.Mol.Biol., 335:519-530, 2004

PubMed Abstract: Mycobacterium tuberculosis, the causative agent of tuberculosis, is known to secrete a number of highly immunogenic proteins that are thought to confer pathogenicity, in part, by mediating binding to host tissues. Among these secreted proteins are the trimeric antigen 85 (Ag85) complex and the related MPT51 protein, also known as FbpC1. While the physiological function of Ag85, a mycolyltransferase required for the biosynthesis of the cell wall component alpha,alpha'-trehalose dimycolate (or cord factor), has been identified recently, the function of the closely related MPT51 (approximately 40% identity with the Ag85 components) remains to be established. The crystal structure of M.tuberculosis MPT51, determined to 1.7 A resolution, shows that MPT51, like the Ag85 components Ag85B and Ag85C2, folds as an alpha/beta hydrolase, but it does not contain any of the catalytic elements required for mycolyltransferase activity. Moreover, the absence of a recognizable alpha,alpha'-trehalose monomycolate-binding site and the failure to detect an active site suggest that the function of MPT51 is of a non-enzymatic nature and that MPT51 may in fact represent a new family of non-catalytic alpha/beta hydrolases. Previous experimental evidence and the structural similarity to some integrins and carbohydrate-binding proteins led to the hypothesis that MPT51 might have a role in host tissue attachment, whereby ligands may include the serum protein fibronectin and small sugars.

PubMed: 14672660
DOI: 10.1016/j.jmb.2003.11.001

実験手法

X-RAY DIFFRACTION (1.71 Å)