1R76

Structure of a pectate lyase from Azospirillum irakense

1R76 の概要

• エントリーDOI: 10.2210/pdb1r76/pdb
• 分子名称: pectate lyase, MERCURY (II) ION, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: a-helical structure, lyase
• 由来する生物種: Azospirillum irakense
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45216.19

構造登録者

Novoa de Armas, H.,Verboven, C.,De Ranter, C.,Desair, J.,Vande Broek, A.,Vanderleyden, J.,Rabijns, A. (登録日: 2003-10-20, 公開日: 2004-06-01, 最終更新日: 2024-02-14)

主引用文献

Novoa De Armas, H.,Verboven, C.,De Ranter, C.,Desair, J.,Vande Broek, A.,Vanderleyden, J.,Rabijns, A.
Azospirillum irakense pectate lyase displays a toroidal fold.
Acta Crystallogr.,Sect.D, 60:999-1007, 2004

PubMed Abstract: The three-dimensional structure of Azospirillum irakense pectate lyase (PelA) has been determined at a resolution of 2.65 A. The crystals are hexagonal, belonging to space group P6(5)22, with unit-cell parameters a = b = 85.37, c = 231.32 angstroms. Phase information was derived from a multiple-wavelength anomalous dispersion (MAD) experiment using a Hg derivative. Refinement of the model converged to Rcryst = 20.08% and Rfree = 25.87%. The overall structure of PelA does not adopt the characteristic parallel beta-helix fold displayed by pectate lyases from polysaccharide lyase (PL) families PL1, PL3 and PL9. Instead, it displays a predominantly alpha-helical structure with irregular coils and short beta-strands, similar to the recently reported structure of the catalytic module of the Cellvibrio japonicus pectate lyase Pel10Acm. The topologies of the two structures have been compared. They show two 'domains' with the interface between them being a wide-open central groove in which the active site is located. The active sites of the crystal structures are also compared and their similarities and differences are discussed.

PubMed: 15159558
DOI: 10.1107/S090744490400602X

実験手法

X-RAY DIFFRACTION (2.65 Å)