1R4Z

Bacillus subtilis lipase A with covalently bound Rc-IPG-phosphonate-inhibitor

Summary for 1R4Z

• Entry DOI: 10.2210/pdb1r4z/pdb
• Related: 1I6W 1R50
• Descriptor: Lipase, [(4R)-2,2-DIMETHYL-1,3-DIOXOLAN-4-YL]METHYL HYDROGEN HEX-5-ENYLPHOSPHONATE (3 entities in total)
• Functional Keywords: lipase, alpha/beta hydrolase, phosphonate inhibitor, hydrolase
• Biological source: Bacillus subtilis
• Cellular location: Secreted: P37957
• Total number of polymer chains: 2
• Total formula weight: 39322.24

Authors

Droege, M.J.,Van Pouderoyen, G.,Vrenken, T.E.,Rueggeberg, C.J.,Reetz, M.T.,Dijkstra, B.W.,Quax, W.J. (deposition date: 2003-10-09, release date: 2004-10-19, Last modification date: 2024-10-30)

Primary citation

Droege, M.J.,Boersma, Y.L.,van Pouderoyen, G.,Vrenken, T.E.,Rueggeberg, C.J.,Reetz, M.T.,Dijkstra, B.W.,Quax, W.J.
Directed Evolution of Bacillus subtilis Lipase A by Use of Enantiomeric Phosphonate Inhibitors: Crystal Structures and Phage Display Selection
Chembiochem, 7:149-157, 2005

PubMed Abstract: Phage display can be used as a protein-engineering tool for the selection of proteins with desirable binding properties from a library of mutants. Here we describe the application of this method for the directed evolution of Bacillus subtilis lipase A, an enzyme that has important properties for the preparation of the pharmaceutically relevant chiral compound 1,2-O-isopropylidene-sn-glycerol (IPG). PCR mutagenesis with spiked oligonucleotides was employed for saturation mutagenesis of a stretch of amino acids near the active site. After expression of these mutants on bacteriophages, dual selection with (S)-(+)- and (R)-(-)-IPG stereoisomers covalently coupled to enantiomeric phosphonate suicide inhibitors (SIRAN Sc and Rc inhibitors, respectively) was used for the isolation of variants with inverted enantioselectivity. The mutants were further characterised by determination of their Michaelis-Menten parameters. The 3D structures of the Sc and Rc inhibitor-lipase complexes were determined and provided structural insight into the mechanism of enantioselectivity of the enzyme. In conclusion, we have used phage display as a fast and reproducible method for the selection of Bacillus lipase A mutant enzymes with inverted enantioselectivity.

PubMed: 16342303
DOI: 10.1002/cbic.200500308

Experimental method

X-RAY DIFFRACTION (1.8 Å)