1R26

Crystal structure of thioredoxin from Trypanosoma brucei brucei

Summary for 1R26

• Entry DOI: 10.2210/pdb1r26/pdb
• Descriptor: Thioredoxin (2 entities in total)
• Functional Keywords: redox-active disulfide; thioredoxin, electron transport
• Biological source: Trypanosoma
• Total number of polymer chains: 1
• Total formula weight: 14483.93

Authors

Friemann, R.,Schmidt, H.,Ramaswamy, S.,Forstner, M.,Krauth-Siegel, R.L.,Eklund, H. (deposition date: 2003-09-26, release date: 2003-12-09, Last modification date: 2024-11-06)

Primary citation

Friemann, R.,Schmidt, H.,Ramaswamy, S.,Forstner, M.,Krauth-Siegel, R.L.,Eklund, H.
Structure of thioredoxin from Trypanosoma brucei brucei
FEBS Lett., 554:301-305, 2003

PubMed Abstract: The three-dimensional structure of thioredoxin from Trypanosoma brucei brucei has been determined at 1.4 A resolution. The overall structure is more similar to that of human thioredoxin than to any other thioredoxin structure. The most striking difference to other thioredoxins is the absence of a buried carboxylate behind the active site cysteines. Instead of the common Asp, there is a Trp that binds an ordered water molecule probably involved in the protonation/deprotonation of the more buried cysteine during catalysis. The conserved Trp in the WCGPC sequence motif has an exposed position that can interact with target proteins.

PubMed: 14623083
DOI: 10.1016/S0014-5793(03)01173-6

Experimental method

X-RAY DIFFRACTION (1.4 Å)