1R1F

Solution Structure of the Cyclotide Palicourein: Implications for the development of pharmaceutical and agricultural applications

1R1F の概要

• エントリーDOI: 10.2210/pdb1r1f/pdb
• 関連するPDBエントリー: 1NB1 1NBJ
• 分子名称: Palicourein (1 entity in total)
• 機能のキーワード: palicourein, cyclotide, plant protein
• 由来する生物種: Palicourea condensata
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3932.45

構造登録者

Barry, D.G.,Daly, N.L.,Bokesch, H.R.,Gustafson, K.R.,Craik, D.J. (登録日: 2003-09-23, 公開日: 2004-04-06, 最終更新日: 2024-11-20)

主引用文献

Barry, D.G.,Daly, N.L.,Bokesch, H.R.,Gustafson, K.R.,Craik, D.J.
Solution structure of the cyclotide palicourein: implications for the development of a pharmaceutical framework.
STRUCTURE, 12:85-94, 2004

PubMed Abstract: The cyclotides are a family of disulfide-rich proteins from plants. They have the characteristic structural features of a circular protein backbone and a knotted arrangement of disulfide bonds. Structural and biochemical studies of the cyclotides suggest that their unique physiological stability can be loaned to bioactive peptide fragments for pharmaceutical and agricultural development. In particular, the cyclotides incorporate a number of solvent-exposed loops that are potentially suitable for epitope grafting applications. Here, we determine the structure of the largest known cyclotide, palicourein, which has an atypical size and composition within one of the surface-exposed loops. The structural data show that an increase in size of a palicourein loop does not perturb the core fold, to which the thermodynamic and chemical stability has been attributed. The cyclotide core fold, thus, can in principle be used as a framework for the development of useful pharmaceutical and agricultural bioactivities.

PubMed: 14725768
DOI: 10.1016/j.str.2003.11.019

実験手法

SOLUTION NMR