1R0N

Crystal Structure of Heterodimeric Ecdsyone receptor DNA binding complex

1R0N の概要

• エントリーDOI: 10.2210/pdb1r0n/pdb
• 関連するPDBエントリー: 1R0O
• 分子名称: Ecdsyone Response Element, Ecdysone Response Element, Retinoic acid receptor RXR-alpha, ... (6 entities in total)
• 機能のキーワード: ecdysone receptor, nuclear receptor, dna binding domain, ultraspiracle, rxr, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P19793 P34021
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 33186.54

構造登録者

Devarakonda, S.,Harp, J.M.,Kim, Y.,Ozyhar, A.,Rastinejad, F. (登録日: 2003-09-22, 公開日: 2003-10-21, 最終更新日: 2024-02-14)

主引用文献

Devarakonda, S.,Harp, J.M.,Kim, Y.,Ozyhar, A.,Rastinejad, F.
Structure of the heterodimeric Ecdysone Receptor DNA-binding complex
Embo J., 22:5827-5840, 2003

PubMed Abstract: Ecdysteroids initiate molting and metamorphosis in insects via a heterodimeric receptor consisting of the ecdysone receptor (EcR) and ultraspiracle (USP). The EcR-USP heterodimer preferentially mediates transcription through highly degenerate pseudo-palindromic response elements, resembling inverted repeats of 5'-AGGTCA-3' separated by 1 bp (IR-1). The requirement for a heterodimeric arrangement of EcR-USP subunits to bind to a symmetric DNA is unusual within the nuclear receptor superfamily. We describe the 2.24 A structure of the EcR-USP DNA-binding domain (DBD) heterodimer bound to an idealized IR-1 element. EcR and USP use similar surfaces, and rely on the deformed minor groove of the DNA to establish protein-protein contacts. As retinoid X receptor (RXR) is the mammalian homolog of USP, we also solved the 2.60 A crystal structure of the EcR-RXR DBD heterodimer on IR-1 and found the dimerization and DNA-binding interfaces to be the same as in the EcR-USP complex. Sequence alignments indicate that the EcR-RXR heterodimer is an important model for understanding how the FXR-RXR heterodimer binds to IR-1 sites.

PubMed: 14592980
DOI: 10.1093/emboj/cdg569

実験手法

X-RAY DIFFRACTION (2.6 Å)