1QTG

AVERAGED NMR MODEL OF SWITCH ARC, A DOUBLE MUTANT OF ARC REPRESSOR

Summary for 1QTG

• Entry DOI: 10.2210/pdb1qtg/pdb
• Related: 1ARR 1PAR
• NMR Information: BMRB: 4540
• Descriptor: Transcriptional repressor arc (1 entity in total)
• Functional Keywords: beta-sheet, right-handed helix, structural change, gene regulation
• Biological source: Lederbergvirus P22
• Total number of polymer chains: 2
• Total formula weight: 12476.54

Authors

Cordes, M.H.J.,Walsh, N.P.,McKnight, C.J.,Sauer, R.T. (deposition date: 1999-06-27, release date: 1999-07-12, Last modification date: 2024-05-22)

Primary citation

Cordes, M.H.,Walsh, N.P.,McKnight, C.J.,Sauer, R.T.
Evolution of a protein fold in vitro.
Science, 284:325-327, 1999

PubMed Abstract: A "switch" mutant of the Arc repressor homodimer was constructed by interchanging the sequence positions of a hydrophobic core residue, leucine 12, and an adjacent surface polar residue, asparagine 11, in each strand of an intersubunit beta sheet. The mutant protein adopts a fold in which each beta strand is replaced by a right-handed helix and side chains in this region undergo significant repacking. The observed structural changes allow the protein to maintain solvent exposure of polar side chains and optimal burial of hydrophobic side chains. These results suggest that new protein folds can evolve from existing folds without drastic or large-scale mutagenesis.

PubMed: 10195898
DOI: 10.1126/science.284.5412.325

Experimental method

SOLUTION NMR