1QSL

KLENOW FRAGMENT COMPLEXED WITH SINGLE-STRANDED SUBSTRATE AND EUROPIUM (III) ION

1QSL の概要

• エントリーDOI: 10.2210/pdb1qsl/pdb
• 分子名称: 5'-D(*GP*CP*TP*TP*AP*CP*GP*C)-3', DNA POLYMERASE I, EUROPIUM ION, ... (4 entities in total)
• 機能のキーワード: exonuclease, two metal ions, single-stranded dna, transferase/dna, transferase-dna complex
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 70748.31

構造登録者

Brautigam, C.A.,Aschheim, K.,Steitz, T.A. (登録日: 1999-06-22, 公開日: 1999-06-30, 最終更新日: 2024-02-14)

主引用文献

Brautigam, C.A.,Aschheim, K.,Steitz, T.A.
Structural elucidation of the binding and inhibitory properties of lanthanide (III) ions at the 3'-5' exonucleolytic active site of the Klenow fragment
Chem.Biol., 6:901-908, 1999

PubMed Abstract: Biochemical and biophysical experiments have shown that two catalytically essential divalent metal ions (termed 'A' and 'B') bind to the 3'-5' exonuclease active site of the Klenow fragment (KF) of Escherichia coli DNA polymerase I. X-ray crystallographic studies have established the normal positions in the KF 3'-5' exonuclease (KF exo) active site of the two cations and the single-stranded DNA substrate. Lanthanide (III) luminescence studies have demonstrated, however, that only a single europium (III) ion (Eu3+) binds to the KF exo active site. Furthermore, Eu3+ does not support catalysis by KF exo or several other two-metal-ion phosphoryl-transfer enzymes.

PubMed: 10631518
DOI: 10.1016/S1074-5521(00)80009-5

実験手法

X-RAY DIFFRACTION (2.2 Å)