1QMW

Solution structure of alpha-conotoxin SI

1QMW の概要

• エントリーDOI: 10.2210/pdb1qmw/pdb
• NMR情報: BMRB: 4503
• 分子名称: ALPHA-CONOTOXIN SI (1 entity in total)
• 機能のキーワード: conotoxin, nicotinic acetylcholine receptor, toxin, venom
• 由来する生物種: CONUS STRIATUS (STRIATED CONE)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1357.64

構造登録者

Benie, A.J.,Whitford, D.,Hargittai, B.,Barany, G.,Janes, R.W. (登録日: 1999-10-08, 公開日: 2000-08-25, 最終更新日: 2024-11-06)

主引用文献

Benie, A.J.,Whitford, D.,Hargittai, B.,Barany, G.,Janes, R.W.
Solution Structure of Alpha-Conotoxin Si
FEBS Lett., 476:287-, 2000

PubMed Abstract: The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solution and crystal structures of alpha-conotoxin GI. Surprisingly, a high degree of similarity between the backbone conformations of the GI crystal and the SI solution structures is seen in the region of lowest sequence homology, namely residues Gly-8 to Ser-12. This similarity is more surprising when considering that in SI a proline replaces the Arg-9 found in GI. The correspondence in conformation in this region provides the definitive evidence that it is the loss of the arginine basic charge at residue 9 which determines the differences in toxicity between GI and SI, rather than any changes in conformation induced by the cyclic proline residue.

PubMed: 10913630
DOI: 10.1016/S0014-5793(00)01724-5

実験手法

SOLUTION NMR