1QM9

NMR, REPRESENTATIVE STRUCTURE

1QM9 の概要

• エントリーDOI: 10.2210/pdb1qm9/pdb
• NMR情報: BMRB: 5409
• 分子名称: POLYPYRIMIDINE TRACT-BINDING PROTEIN (1 entity in total)
• 機能のキーワード: ribonucleoprotein, polypyrimidine tract binding protein, rnp, rna, spicing, translation
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Nucleus: P26599
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22117.32

構造登録者

Conte, M.R.,Grune, T.,Curry, S.,Matthews, S. (登録日: 1999-09-22, 公開日: 2000-07-03, 最終更新日: 2024-05-15)

主引用文献

Conte, M.R.,Grune, T.,Kelly, G.,Ladas, A.,Matthews, S.,Curry, S.
Structure of Tandem RNA Recognition Motifs from Polypyrimidine Tract Binding Protein Reveals Novel Features of the Rrm Fold
Embo J., 19:3132-, 2000

PubMed Abstract: Polypyrimidine tract binding protein (PTB), an RNA binding protein containing four RNA recognition motifs (RRMs), is involved in both pre-mRNA splicing and translation initiation directed by picornaviral internal ribosome entry sites. Sequence comparisons previously indicated that PTB is a non-canonical RRM protein. The solution structure of a PTB fragment containing RRMs 3 and 4 shows that the protein consists of two domains connected by a long, flexible linker. The two domains tumble independently in solution, having no fixed relative orientation. In addition to the betaalphabetabetaalphabeta topology, which is characteristic of RRM domains, the C-terminal extension of PTB RRM-3 incorporates an unanticipated fifth beta-strand, which extends the RNA binding surface. The long, disordered polypeptide connecting beta4 and beta5 in RRM-3 is poised above the RNA binding surface and is likely to contribute to RNA recognition. Mutational analyses show that both RRM-3 and RRM-4 contribute to RNA binding specificity and that, despite its unusual sequence, PTB binds RNA in a manner akin to that of other RRM proteins.

PubMed: 10856256
DOI: 10.1093/EMBOJ/19.12.3132

実験手法

SOLUTION NMR