1QL5

DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT

Summary for 1QL5

• Entry DOI: 10.2210/pdb1ql5/pdb
• NMR Information: BMRB: 4412
• Descriptor: DNA (5'-D(*CP*GP*CP*AP*TP*+TP*AP*CP*GP*C)- 3'), DNA (5'-D(*GP*CP*GP*TP*TP*AP*TP*GP*CP*G)-3') (2 entities in total)
• Functional Keywords: dna, deoxyribonucleic acid, dna photoproduct, (6-4) adduct, mutagenesis
• Total number of polymer chains: 2
• Total formula weight: 6085.03

Authors

Lee, J.-H.,Hwang, G.-S.,Choi, B.-S. (deposition date: 1999-08-24, release date: 2000-04-10, Last modification date: 2024-05-15)

Primary citation

Lee, J.-H.,Choi, Y.-J.,Choi, B.-S.
Solution Structure of a DNA Decamer Duplex Containing the 3' T.T Base Pair of the Cis-Syn Cyclobutane Pyrimidine Dimer: Implication for the Mutagenic Property of the Cis-Syn Dimer.
Nucleic Acids Res., 28:1794-, 2000

PubMed Abstract: The cis - syn dimer is the major DNA photoproduct produced by UV irradiation. In order to determine the origin of the mutagenic property of the cis - syn dimer, we used NMR restraints and molecular dynamics to determine the solution structure of a DNA decamer duplex containing a wobble pair between the 3'-T of the cis - syn dimer and the opposite T residue (CS/TA duplex). The solution structure of the CS/TA duplex revealed that the 3'-T x T base pair of the cis - syn dimer had base pair geometry that was significantly different from the canonical Watson-Crick base pair and caused destabilization and conformational distortion of its 3'-region. However, a 3'-T x A base pair at the cis - syn dimer within this related DNA decamer maintains the normal Watson-Crick base pair geometry and causes little distortion in the conformation of its 3'-side. Our results show that in spite of its stable hydrogen bonding, the insertion of a T residue opposite the 3'-T of the cis - syn dimer is inhibited by structural distortion caused by the 3'-T x T base pair. This may explain why the frequency of the 3'-T-->A transversion, which is the major mutation produced by the cis - syn dimer, is only 4%.

PubMed: 10734199
DOI: 10.1093/NAR/28.8.1794

Experimental method

SOLUTION NMR