1QKW

Alpha-spectrin Src Homology 3 domain, N47G mutant in the distal loop.

1QKW の概要

• エントリーDOI: 10.2210/pdb1qkw/pdb
• 関連するPDBエントリー: 1AEY 1AJ3 1BK2 1SHG 1TUC 1TUD
• 分子名称: ALPHA II SPECTRIN, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: cytoskeleton, membrane, sh3 domain
• 由来する生物種: GALLUS GALLUS (CHICKEN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7344.24

構造登録者

Vega, M.C.,Martinez, J.,Serrano, L. (登録日: 1999-08-16, 公開日: 2000-08-18, 最終更新日: 2023-12-13)

主引用文献

Vega, M.C.,Martinez, J.C.,Serrano, L.
Thermodynamic and structural characterization of Asn and Ala residues in the disallowed II' region of the Ramachandran plot.
Protein Sci., 9:2322-2328, 2000

PubMed Abstract: Residue Asn47 at position L1 of a type II' beta-turn of the alpha-spectrin SH3 domain is located in a disallowed region of the Ramachandran plot (phi = 56 +/- 12, psi = -118 +/- 17). Therefore, it is expected that replacement of Asn47 by Gly should result in a considerable stabilization of the protein. Thermodynamic analysis of the N47G and N47A mutants shows that the change in free energy is small (approximately 0.7 kcal/mol; approximately 3 kJ/mol) and comparable to that found when mutating a Gly to Ala in a alpha-helix or beta-sheet. X-ray structural analysis of these mutants shows that the conformation of the beta-turn does not change upon mutation and, therefore, that there is no relaxation of the structure, nor is there any gain or loss of interactions that could explain the small energy change. Our results indicate that the energetic definition of II' region of the Ramachandran plot (phi = 60 +/- 30, psi = -115 +/- 15) should be revised for at least Ala and Asn in structure validation and protein design.

PubMed: 11206053
DOI: 10.1110/ps.9.12.2322

実験手法

X-RAY DIFFRACTION (2 Å)