1QK7

Solution structure of Selenocosmia huwena lectin-I(SHL-I) by 2D-NMR

1QK7 の概要

• エントリーDOI: 10.2210/pdb1qk7/pdb
• 分子名称: SELENOCOSMIA HUWENA LECTIN-I (1 entity in total)
• 機能のキーワード: lectin, selenocosmia huwena, shl-i, cystine knot
• 由来する生物種: SELENOCOSMIA HUWENA (CHINESE BIRD SPIDER)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3549.07

構造登録者

Lu, S.,Liang, S.,Gu, X. (登録日: 1999-07-10, 公開日: 1999-08-20, 最終更新日: 2024-11-20)

主引用文献

Lu, S.,Liang, S.,Gu, X.
Three Dimensional Structure of Selenocosmia Huwena Lectin-I (Shl-I) from the Venom of the Spider Selenocosmia Huwena by 2D-NMR
J.Protein Chem., 18:609-, 2000

PubMed Abstract: The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.

PubMed: 10524779
DOI: 10.1023/A:1020663619657

実験手法

SOLUTION NMR