1QHW

PURPLE ACID PHOSPHATASE FROM RAT BONE

Summary for 1QHW

• Entry DOI: 10.2210/pdb1qhw/pdb
• Descriptor: PROTEIN (PURPLE ACID PHOSPHATASE), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, FE (III) ION, ... (6 entities in total)
• Functional Keywords: metal phosphatase, hydrolase
• Biological source: Rattus norvegicus (Norway rat)
• Cellular location: Lysosome: P29288
• Total number of polymer chains: 1
• Total formula weight: 37629.02

Authors

Lindqvist, Y.,Johansson, E.,Kaija, H.,Vihko, P.,Schneider, G. (deposition date: 1999-03-26, release date: 1999-09-15, Last modification date: 2024-11-13)

Primary citation

Lindqvist, Y.,Johansson, E.,Kaija, H.,Vihko, P.,Schneider, G.
Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center.
J.Mol.Biol., 291:135-147, 1999

PubMed Abstract: The crystal structure of purple acid phosphatase from rat bone has been determined by molecular replacement and the structure has been refined to 2.2 A resolution to an R -factor of 21.3 % (R -free 26.5 %). The core of the enzyme consists of two seven-stranded mixed beta-sheets, with each sheet flanked by solvent-exposed alpha-helices on one side. The two sheets pack towards each other forming a beta-sandwich. The di-iron center, located at the bottom of the active-site pocket at one edge of the beta-sandwich, contains a mu-hydroxo or mu-oxo bridge and both metal ions are observed in an almost perfect octahedral coordination geometry. The electron density map indicates that a mu-(hydr)oxo bridge is found in the metal center and that at least one solvent molecule is located in the first coordination sphere of one of the metal ions. The crystallographic study of rat purple acid phosphatase reveals that the mammalian enzymes are very similar in overall structure to the plant enzymes in spite of only 18 % overall sequence identity. In particular, coordination and geometry of the iron cluster is preserved in both enzymes and comparison of the active-sites suggests a common mechanism for the mammalian and plant enzymes. However, significant differences are found in the architecture of the substrate binding pocket.

PubMed: 10438611
DOI: 10.1006/jmbi.1999.2962

Experimental method

X-RAY DIFFRACTION (2.2 Å)