1QHW

PURPLE ACID PHOSPHATASE FROM RAT BONE

1QHW の概要

• エントリーDOI: 10.2210/pdb1qhw/pdb
• 分子名称: PROTEIN (PURPLE ACID PHOSPHATASE), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, FE (III) ION, ... (6 entities in total)
• 機能のキーワード: metal phosphatase, hydrolase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Lysosome: P29288
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37629.02

構造登録者

Lindqvist, Y.,Johansson, E.,Kaija, H.,Vihko, P.,Schneider, G. (登録日: 1999-03-26, 公開日: 1999-09-15, 最終更新日: 2024-11-13)

主引用文献

Lindqvist, Y.,Johansson, E.,Kaija, H.,Vihko, P.,Schneider, G.
Three-dimensional structure of a mammalian purple acid phosphatase at 2.2 A resolution with a mu-(hydr)oxo bridged di-iron center.
J.Mol.Biol., 291:135-147, 1999

PubMed Abstract: The crystal structure of purple acid phosphatase from rat bone has been determined by molecular replacement and the structure has been refined to 2.2 A resolution to an R -factor of 21.3 % (R -free 26.5 %). The core of the enzyme consists of two seven-stranded mixed beta-sheets, with each sheet flanked by solvent-exposed alpha-helices on one side. The two sheets pack towards each other forming a beta-sandwich. The di-iron center, located at the bottom of the active-site pocket at one edge of the beta-sandwich, contains a mu-hydroxo or mu-oxo bridge and both metal ions are observed in an almost perfect octahedral coordination geometry. The electron density map indicates that a mu-(hydr)oxo bridge is found in the metal center and that at least one solvent molecule is located in the first coordination sphere of one of the metal ions. The crystallographic study of rat purple acid phosphatase reveals that the mammalian enzymes are very similar in overall structure to the plant enzymes in spite of only 18 % overall sequence identity. In particular, coordination and geometry of the iron cluster is preserved in both enzymes and comparison of the active-sites suggests a common mechanism for the mammalian and plant enzymes. However, significant differences are found in the architecture of the substrate binding pocket.

PubMed: 10438611
DOI: 10.1006/jmbi.1999.2962

実験手法

X-RAY DIFFRACTION (2.2 Å)