1QH2

CHYMOTRYPSIN INHIBITOR (C2) FROM NICOTIANA ALATA

1QH2 の概要

• エントリーDOI: 10.2210/pdb1qh2/pdb
• 分子名称: PROTEIN (TRYPSIN INHIBITOR C2) (2 entities in total)
• 機能のキーワード: proteinase inhibitor (chymotrypsin), serine proteinase inhibitor, potato ii trypsin inhibitor, nicotiana alata, hydrolase inhibitor
• 由来する生物種: Nicotiana alata (Persian tobacco); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5023.82

構造登録者

Lee, M.C.S.,Scanlon, M.J.,Anderson, M.A.,Craik, D.J. (登録日: 1999-05-11, 公開日: 1999-05-24, 最終更新日: 2024-11-13)

主引用文献

Lee, M.C.,Scanlon, M.J.,Craik, D.J.,Anderson, M.A.
A novel two-chain proteinase inhibitor generated by circularization of a multidomain precursor protein.
Nat.Struct.Biol., 6:526-530, 1999

PubMed Abstract: Female reproductive tissues of the ornamental tobacco amass high levels of serine proteinase inhibitors (PIs) for protection against pests and pathogens. These PIs are produced from a precursor protein composed of six repeats each with a protease reactive site. Here we show that proteolytic processing of the precursor generates five single-chain PIs and a remarkable two-chain inhibitor formed by disulfide-bond linkage of N- and C-terminal peptide fragments. Surprisingly, PI precursors adopt this circular structure regardless of the number of inhibitor domains, suggesting this bracelet-like conformation is characteristic of the widespread potato inhibitor II (Pot II) protein family.

PubMed: 10360353
DOI: 10.1038/9293

実験手法

SOLUTION NMR