1QFL

BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH A REACTION INTERMEDIATE.

1QFL の概要

• エントリーDOI: 10.2210/pdb1qfl/pdb
• 分子名称: PROTEIN (ACETOACETYL-COA THIOLASE), SULFATE ION, COENZYME A, ... (4 entities in total)
• 機能のキーワード: thiolase, coa, tetramerization motif, covalent intermediate, acetyl-cysteine, transferase
• 由来する生物種: Zoogloea ramigera
• 細胞内の位置: Cytoplasm: P07097
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 164838.31

構造登録者

Modis, Y.,Wierenga, R.K. (登録日: 1999-04-12, 公開日: 2000-04-19, 最終更新日: 2024-10-30)

主引用文献

Modis, Y.,Wierenga, R.K.
A biosynthetic thiolase in complex with a reaction intermediate: the crystal structure provides new insights into the catalytic mechanism.
Structure Fold.Des., 7:1279-1290, 1999

PubMed Abstract: Thiolases are ubiquitous and form a large family of dimeric or tetrameric enzymes with a conserved, five-layered alphabetaalphabetaalpha catalytic domain. Thiolases can function either degradatively, in the beta-oxidation pathway of fatty acids, or biosynthetically. Biosynthetic thiolases catalyze the biological Claisen condensation of two molecules of acetyl-CoA to form acetoacetyl-CoA. This is one of the fundamental categories of carbon skeletal assembly patterns in biological systems and is the first step in a wide range of biosynthetic pathways, including those that generate cholesterol, steroid hormones, and various energy-storage molecules.

PubMed: 10545327
DOI: 10.1016/S0969-2126(00)80061-1

実験手法

X-RAY DIFFRACTION (1.92 Å)